guy salvesen (Addgene inc)
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guy salvesen
Guy Salvesen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/guy+salvesen/pcDNA3+HA-Sumo2+WT+(Plasmid+%2348967)/10__1016_slash_j__ijbiomac__2025__146632-192-8-10
Average 93 stars, based on 18 article reviews
Guy Salvesen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/guy+salvesen/pcDNA3+HA-Sumo2+WT+(Plasmid+%2348967)/10__1016_slash_j__ijbiomac__2025__146632-192-8-10
Average 93 stars, based on 18 article reviews
guy salvesen - by Bioz Stars,
2026-09
93/100 stars
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Concentration Assay:Article Title: Lactate regulates cell cycle by remodeling the anaphase promoting complex Article Snippet: .. The eluted proteins were then concentrated using a 3k cutoff Amicon Ultra-15 Centrifugal Filter Units and after measuring the concentration using Pierce BCA protein assay kit, 6×His-UBE2C was then frozen at −80°C. pET28a-SENP1 (catalytic domain) was a gift from Bicinchoninic Acid Protein Assay:Article Title: Lactate regulates cell cycle by remodeling the anaphase promoting complex Article Snippet: .. The eluted proteins were then concentrated using a 3k cutoff Amicon Ultra-15 Centrifugal Filter Units and after measuring the concentration using Pierce BCA protein assay kit, 6×His-UBE2C was then frozen at −80°C. pET28a-SENP1 (catalytic domain) was a gift from Plasmid Preparation:Article Title: Structural basis for the human SENP5's SUMO isoform discrimination. Article Snippet: .. FulllengthRGS-SENP5was a gift fromEdwardYeh (Addgeneplasmid#18053; http://n2t.net/addgene:18053; RRID:Addgene_18053), pLPC-Flag-PML-IV was a gift from Gerardo Ferbeyre (Addgene plasmid #62804; http://n2t. net/addgene:62804; RRID:Addgene_62804), pcDNA3-HA-SUMO1 was a gift from JunyingYuan (Addgeneplasmid#21154;http://n2t.net/addgene: 21154; RRID:Addgene_21154) and pcDNA3HA-SUMO2WTwas a gift from Article Title: Structural basis for the human SENP5’s SUMO isoform discrimination Article Snippet: .. Full-length RGS-SENP5 was a gift from Edward Yeh (Addgene plasmid #18053; http://n2t.net/addgene:18053 ; RRID:Addgene_18053), pLPC-Flag-PML-IV was a gift from Gerardo Ferbeyre (Addgene plasmid #62804; http://n2t.net/addgene:62804 ; RRID:Addgene_62804), pcDNA3-HA-SUMO1 was a gift from Junying Yuan (Addgene plasmid #21154; http://n2t.net/addgene:21154 ; RRID:Addgene_21154) and pcDNA3 HA-SUMO2 WT was a gift from Article Title: SUMO-2 activity is inhibited by non-covalent interactions with the Aβ peptide: an exploration of potential pathogenic mechanisms in Alzheimer's disease Article Snippet: SUMOylation is a post-translational modification involving the addition of SUMO isoforms to target proteins and plays a role in various biological processes, including neurodegenerative diseases and ocular pathologies.. This study investigates the interaction between SUMO-2 and amyloid (Aβ) peptides, key contributors to Alzheimer’s disease, using techniques like cross-linking mass spectrometry, surface plasmon resonance and biolayer interferometry.. Data are available via ProteomeXchange with identifier PXD066055. Construct:Article Title: Lactate regulates cell cycle by remodeling the anaphase promoting complex Article Snippet: 15 N-labeled SENP1 was purified as described above. pET28a-HsSENP3 (307–574, catalytic domain) (Addgene 71481) was a gift from Jorge Eduardo Azevedo. pET28a-SENP5 (catalytic domain) (Addgene 16358) was a gift from Guy Salvesen. .. The N-terminal His tag constructs of human SENP3 and SENP5 were expressed and purified as described above for SENP1, except Tris (pH 8.0) buffers were used for Ni-affinity step in place of HEPES (pH 7.5) and thrombin cleavage step was omitted. pET28a-SENP2/7 (catalytic domains) were gifts from Purification:Article Title: Lactate regulates cell cycle by remodeling the anaphase promoting complex Article Snippet: 15 N-labeled SENP1 was purified as described above. pET28a-HsSENP3 (307–574, catalytic domain) (Addgene 71481) was a gift from Jorge Eduardo Azevedo. pET28a-SENP5 (catalytic domain) (Addgene 16358) was a gift from Guy Salvesen. .. The N-terminal His tag constructs of human SENP3 and SENP5 were expressed and purified as described above for SENP1, except Tris (pH 8.0) buffers were used for Ni-affinity step in place of HEPES (pH 7.5) and thrombin cleavage step was omitted. pET28a-SENP2/7 (catalytic domains) were gifts from |